Factors Affection Plaque Formation
- Factors affecting plaque formation
- Electrolyte requirements
- Use of indicator strains in phage assay
- Growth phase of host
- Influence of growth medium
- Phage diluent
- Temperature
- Hydrogen-ion concentration
- Broth or milk-grown cells in phage assays
- Raw milk phages
- Need for the individual component strains as hosts in plaque assay
- Incorporation of glycine in growth media
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The major factors that influence plaque formation by lytic lactococcal phages were reviewed by the author in 1979, and in 1986. These papers and the references to the literature can be downloaded by the author's students, this facility is currently not available to other site users. Work reported by Dag Lillehaug (1996) who has studied plaque formation by six lysogenic lactococcal phages is also briefly summarised. Dag Lillehaug's paper is available as a free download from the publisher's website.
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