Calculator for determining the time required to reduce an initial population of a microorganism to a predetermined number of survivors

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Calculator for determining the time required to reduce an initial population of a microorganism to a predetermined number of survivors

To use the calculator replace the initial values with your data.
Initial numbers of organism (CFU/mL)	e.g.100 CFU/mL
D₇₂ value (seconds) of organism	e.g. 14 seconds
Number of surviving organisms (CFU/mL)	e.g. 0.1 CFU/mL

NOTE! The science and mathematics behind this calculator are described in the modeling exercise to investigate the survival of Mycobacterium avium subsp. paratuberculosis following HTST treatment of milk.

How to cite this article

Mullan, W.M.A. (2010). [On-line]. Available: Accessed:

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