Table 1. Identification of Enterococcus species with a biochemical keya devised by Manero and Blanch (1999)

Test or characteristic

E. asini

E. avium

E. casseliflavus

E. durans

E. faecalis

E. faecium

E. gallinarum

E. hirae

E. malodoratus

E. mundtii

E. pseudoavium

E. solitarius

E. raffinosus

E. cecorum

E. dispar

E. saccharolyticus

E. sulfureus

E. columbae

E. flavescens

Metabolism with:

                                     

 N-Acetylglucosamine

+

+

+

+

+

+

+

+

+

+

+

+

+

+

ND

ND

+

+

+

Adonitol

+

+

+

d

Amygdalin

+

+

+

+

+

+

+

+

+

+

+

+

ND

+

ND

ND

+

V

+

l-Arabinose

+

+

+

+

+

+

+

+

d-Arabitol

+

+

ND

+

d

+

V

l-Arabitol

d

+

+

+

Arbutin

+

+

+

+

+

+

+

+

+

ND

+

ND

+

ND

ND

+

+

+

Cellobiose

+

+

+

+

+

+

+

+

+

+

+

+

ND

+

ND

ND

+

+

+

Dextrin

ND

(+)

+

ND

+

V

+

ND

ND

ND

ND

ND

ND

ND

ND

ND

ND

ND

ND

Dulcitol

d

d

ND

Erythritol

ND

ND

ND

ND

ND

ND

d-Fructose

+

+

+

+

+

+

+

+

+

+

ND

+

ND

+

ND

ND

+

+

+

d-Fucose

ND

ND

ND

ND

ND

l-Fucose

ND

ND

ND

ND

ND

Galactose

+

+

+

+

+

+

+

+

+

+

+

+

ND

+

ND

ND

+

+

+

Gluconate

(+)

+

(+)

V

+

+

ND

+

ND

d

+

+

d-Glucose

+

+

+

+

+

+

+

+

+

+

ND

ND

ND

+

ND

ND

+

+

ND

Glycerol

d

V

+

d

d

d

d

d

+

+

+

Glycogen

d

(−)

ND

Inulin

d

(+)

d

d

d

+

+

+

+

2-Ketogluconate

+

V

+

+

+

V

+

+

+

(−)

5-Ketogluconate

V

ND

ND

V

ND

ND

ND

Lactose

+

+

+

+

+

+

+

+

+

+

+

+

+

+

ND

+

(+)

+

d-Lyxose

+

ND

ND

Maltose

+

+

+

+

+

+

+

+

+

+

+

+

ND

+

ND

ND

+

+

+

Mannitol

+

+

+

+

+

+

+

+

+

+

d

+

+

+

d-Mannose

+

+

+

+

+

+

+

+

+

+

ND

+

+

+

ND

ND

+

+

+

Melibiose

d

+

d

(+)

+

+

+

+

(−)

+

+

d

+

+

+

ND

Melezitose

+

d

(+)

d

d

+

+

d

+

+

(−)

Methyl-α-d-glucopyranoside

(−)

+

+

(−)

+

d

+

+

+

d

+

+

+

+

+

Methyl-α-d-mannopyranoside

V

V

(−)

(−)

+

(+)

ND

ND

ND

ND

+

Methyl xyloside

ND

ND

ND

ND

ND

ND

d-Raffinose

d

d

+

+

d

+

(+)

+

+

+

+

+

+

Rhamnose

+

+

(+)

v

+

+

+

ND

ND

(−)

+

Ribose

+

+

+

+

+

+

+

+

+

+

+

+

+

ND

+

+

Salicin

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

Sorbitol

+

d

+

d

d

d

+

d

+

d

+

+

Sorbose

+

+

+

ND

+

ND

Starch

(+)

V

d

d

d

d

(+)

ND

d

V

+

ND

ND

+

Sucrose

(+)

+

d

+

+

+

+

+

+

d

+

+

+

+

ND

+

+

+

d-Tagatose

+

+

+

(−)

d

+

+

ND

ND

V

Trehalose

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

ND

+

+

+

d-Turanose

d

V

+

(−)

V

ND

+

ND

ND

ND

ND

(−)

l-Xylose

d

(−)

V

ND

ND

ND

ND

ND

ND

d-Xylose

+

d

+

V

d

+

V

+

ND

ND

+

+

Xylitol

+

+

ND

ND

(−)

Growth at:

                                     

4C

ND

ND

ND

+

ND

ND

ND

ND

ND

ND

ND

ND

ND

ND

ND

ND

10C

V

d

+

+

+

+

+

+

+

+

+

(+)

+

+

+

(−)

45C

V

+

(+)

+

+

+

+

+

+

+

+

+

+

+

ND

(+)

50C

ND

V

ND

ND

ND

ND

ND

ND

ND

pH 9.6

ND

+

+

+

+

+

+

+

ND

+

+

ND

+

ND

ND

ND

ND

ND

ND

Growth in:

                                     

6.5% NaCl

d

+

+

+

+

+

+

+

+

d

+

+

+

+

+

ND

0.1% Methylene blue milk

ND

ND

+

+

+

V

ND

ND

ND

ND

ND

ND

V

ND

ND

ND

ND

ND

0.04% Tellurite

ND

d

+

d

d

ND

ND

ND

ND

ND

ND

0.01% Tetrazolium

ND

ND

ND

+

+

ND

ND

ND

ND

ND

ND

ND

ND

ND

ND

ND

ND

Survival at 60C for:

                                     

15 min

ND

+

ND

ND

+

+

(+)

ND

ND

ND

ND

ND

ND

ND

ND

ND

ND

ND

ND

30 min

+

+

ND

ND

+

+

d

ND

ND

ND

ND

ND

ND

ND

ND

ND

ND

ND

1 h

ND

(−)

ND

ND

+

+

ND

ND

ND

ND

ND

ND

ND

ND

ND

ND

ND

ND

Gelatin liquefaction

ND

d

ND

ND

ND

ND

ND

ND

ND

ND

H2S production

ND

d

ND

+

ND

ND

ND

ND

ND

ND

ND

ND

ND

ND

Alpha hemolysis

ND

+

+

V

V

d

d

d

d

d

ND

ND

ND

ND

Beta hemolysis

ND

V

V

d

d

d

ND

ND

ND

ND

Lancefield group D

+

+

+

(+)

+

V

+

V

+

+

+

ND

+

Motility

+

+

ND

+

Voges-Proskauer

ND

d

d

+

+

+

d

+

d

+

d

d

d

d

ND

ND

+

+

Yellow pigment

+

+

+

+

Alkaline phosphatase

ND

d

(−)

+

+

Arginine dihydrolase

d

+

+

+

+

+

+

+

+

+

Catalase

Esculin hydrolysis

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

α-Galactosidase

+

d

+

+

+

(+)

d

+

d

d

+

+

+

+

+

β-Galactosidase

d

+

d

d

+

+

(+)

+

+

d

d

+

ND

+

V

+

β-Glucoronidase

d

+

ND

Hippurate hydrolysis

+

d

V

d

d

d

d

d

d

d

d

d

V

Leucine arylamidase

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

Pyrrolidonyl aminopeptidase

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

a+, 90% or more of the strains of isolates are positive; (+), 75 to 89% are positive; V, 26 to 74% are positive; (−), 11 to 25% are positive; −, 10 or less are positive; ND, no data;

From: Manero, A. and. Blanch, A.R. (1999). Identification of Enterococcus spp. with a biochemical key. Applied Environmental Microbiology 65, 44254430.
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