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Calculate the number of samples required to assure the absence of a pathogen or fault-causing organism at a selected probability

To use add your values (numerical only) to the empty fields below.


Probability Value is normally 0.95 (95%)

What mass (g) or volume (mL) of food should be free of the organism?

e.g. 500 g
Mass or volume of samples used e.g. 25 g

Note this calculator is part of a tutorial/workshop on the: Reliability of microbial sampling in assuring food safety.

Caution. This free calculator is intended for educational use only and is not a substitute for a company employing a qualified food technologist or microbiologist. It assumes that microorganisms present are homogeneously distributed.

How to cite this article

Mullan, W.M.A. (2020) [On-line] Available from: Accessed: